Figure 7
Aberrant replication dynamics in fetal liver cells in the absence of Cdh1. (A) Schematic drawing of a DNA fiber after pulse labeling with CldU (red) and IdU (green). Green track measurements allow quantification of fork speed. Origins are identified based on the pattern of green and red staining and the distance between two adjacent origins on the same DNA fiber defines IOD. (B) Replication fork rates were measured as detailed in (A) on stretched DNA fibers obtained from E14.5 fetal liver cells of the indicated genotype (n = 607 for WT and 601 for KO). (C) Representative immunofluorescence images of stretched DNA fibers from the indicated fetal liver cells showing CldU (red) and IdU (green) staining. (D) Distribution of IOD values from the same samples as in (B) (n = 50 for WT and 51 for KO). In scatter dot plots in (B) and (D) the black line indicates median value for the plotted data. ***P < 0.001, ****P < 0.0001.
