Figure 1

Transcriptomic analysis of MUNASQA and TJ2049 genotypes under drought. Heat-map of all DEGs for MUNASQA and TJ2049 in drought conditions. Scale color indicates green for up-regulation and red for downregulation (a). GO enrichment in MUNASQA and TJ2049 comprises biological processes (BP, in red), molecular function (MF, in blue), and cellular component (CC in green). Relevant categories showing enrichment of DEGs for both genotypes are depicted. GO terms were plotted after applying an FDR = 0.1 Bubble size correlates with enrichment factor values; for each bubble size, the P-value is indicated (b). Venn diagram for all DEGs in MUNASQA and TJ2049 under drought conditions. DEGs were plotted after applying an FDR = 0.1 (c). Validation by qRT-PCR of ten genes selected from RNA-Seq. Log₂ fold change (log2FC) was calculated based on the comparison of drought vs control for each genotype (d). Three biological replicates were used, and the experiment was performed twice with similar results.