Figure 1 | Scientific Reports

Figure 1

From: Visualization of learning-induced synaptic plasticity in output neurons of the Drosophila mushroom body γ-lobe

Figure 1

Visualization of postsynaptic calcium in the mushroom body output neurons. (a) Mushroom body structure and compartmentalization of the γ-lobe. (b) Functional compartmentalization of the γ-lobe, indicating the connectivity between odor-encoding Kenyon cells (KCs), punishminent- or reward-signaling dopaminergic neurons (DANs) and behavior-instructive mushroom body oitput neurons (MBONs). (c) Putative connectivity within the Kenyon cell (KC)-dopaminergic neuron (DAN)-mushroom body output neuron (MBON) microcircuit in the γ-lobe. (d) Representative confocal images showing the expression pattern of cytosolic sensors GCaMP6f and GCaMP3, and postsynaptically localized homer-GCaMP across the MBONs of the γ-lobe. (e–g) Examples of functional imaging recordings using cytosolic GCaMP6f and GCaMP3 and postsynaptically localized homer-GCaMP. Line graphs show mean ΔF/F0 values and SEM. Grey bars indicate the odor presentation period for the odorants 4-methylcyclohexanol (MCH) and 3-octanol (OCT). For GCaMP6f, n = 119 flies, for GCaMP3, n = 12 flies, for homer-GCaMP, n = 30 flies.

Back to article page