Figure 3
Induction and visualization of learning-induced plasticity at the MBON-γ1pedc > α/β postsynapse. (a) Imaging procedures used to probe the effects of associative conditioning on MBON odor responses. Flies were exposed to one of three protocols: (1) aversive associative conditioning; (2) ‘odor only’ control; or (3) ‘shock only’ control. Before and after the training procedure, three odors were presented and MBON postsynaptic calcium was monitored using homer-GCaMP. (b) Schematic highlighting the γ1 compartment in which MBON-γ1pedc > α/β postsynaptic sites were imaged. (c–e) MBON-γ1pedc > α/β odor responses measured before (pre-training) and after (post-training) one of the three training protocols. Lines indicate means, and shaded areas represent SEMs. The black line in each case represents the pre-training response. Post-training responses are shown in color, dependent on condition. The yellow box indicates the period of odor presentation. The area under the curve (AUC) was calculated over the 5 s after odor onset, and pre- to post-training differences were tested using Wilcoxon signed rank tests (*p < 0.05). Boxes represent 25% and 75% quartiles, squares indicate means, and horizontal lines indicate medians. Whiskers show minimum and maximum values. For the paired group (c), n = 9 flies. For both control groups (d, e), n = 10 flies.
