Figure 6
MBONs innervating the γ1 and γ5 mushroom body compartments are required for conditioned odor avoidance. (a) Training and test procedures. Training refers to aversive associative conditioning procedure schematized in Fig. 3. Temperature-sensititive shibire expression was used to block synaptic vesicle exocytosis. Upper panel shows the control condition in which training and test were carried out at permissive temperature. Lower panel shows experimental procedure in which training and test were carried out at restrictive temperature. (b–f) Box plots showing learning indices when blocking synaptic output from different MBON types ((b) MBON-γ1pedc > α/β; (c) MBON-γ2α′2; (d) MBON-γ3, MBON-γ3β′1; (e) MBON-γ4 > γ1γ2; (f) MBON-γ5β′2a, MBON-β′2mp). Boxes represent 25% and 75% quartiles, squares indicate means, and horizontal lines indicate medians. Whiskers show minimum and maximum values. For all groups, n = 8. Difference between groups were tested using one-way ANOVA with Tukey post-hoc tests.
