Figure 1
From: Smurf2 inhibition enhances chemotherapy and radiation sensitivity in non-small-cell lung cancer
Proliferation and apoptosis analyses of PC9 and A549 NSCLC cells with siRNA-mediated Smurf2 knockdown. Western blot analysis of total Smurf2 and SMAD1 protein levels (A) in PC9 and A549 cells after Smurf2 knockdown was confirmed in siRNA-transfected PC9 cells relative to PC9 cells transfected with nontargeting (NT) control siRNA. GAPDH was used as the loading control. Significantly decreased cell survival was observed by MTT assay in Smurf2-knockdown PC9 cells compared to NT siRNA-transfected cells after treatment with increasing concentrations of cisplatin and doses of radiation (RT) (B). Quantitative determination of apoptosis in siRNA-mediated Smurf2-knockdown vs. NT siRNA-transfected tumor cells by flow cytometry after treatment with increasing concentrations of cisplatin and doses of radiation (C). PC9 and A549 cells were colabeled with FITC-conjugated annexin V (AV) and PI. *p < 0.05; **p < 0.01.
