Figure 4
From: Streamlined and quantitative detection of chimerism using digital PCR
Validation in various organs and tissues. (a) Flow cytometry plot of a CD1 and BL6 mouse peripheral blood mixture stained with CD45.1-PE-Cy7 (y-axis, CD1 strain) and CD45.2-BV421 (x-axis, BL6 strain) antibodies. (b) Bottom: CD1 and BL6 blood mixed at different volumetric ratios. Top: Measured chimerism of CD1 and BL6 blood mixtures. Measured by FCM using CD45.1/CD45.2 antibodies (dashed lines) or SND-ddPCR assay (solid lines). (c) Chimerism was measured with crude lysates of various organs from 5 chimeric mice (CD1:BL6 chimeras). CD1, BL6 and CD1xDBA2J F1 hybrid are non-chimeric controls. (d) Number of BL6 and CD1 cells in blastocysts (1 lysed blastocyst per reaction). 4 CD1, 4 BL6 and 4 chimeric blastocysts (CD1 injected with BL6 embryonic stem cells) were analyzed.
