Figure 2 | Scientific Reports

Figure 2

From: The cell cycle stage of bovine zygotes electroporated with CRISPR/Cas9-RNP affects frequency of Loss-of-heterozygosity editing events

Figure 2

(A) Sequence details of all Homozygous Full edit editing events (n = 21) represented by a total of 15 INDEL editing variants. The probability of Loss-of-heterozygosity for of all these 15 INDEL variants involving both homologous chromosomes is far above expected plausibility based on likelihood of observed INDELS in single chromosomes suggesting a distinct mechanistic cause rather than random effects. (B) Effects of early (n = 131) and late (n = 213) electroporation (10 vs. 18 HPI) on mosaic edit to all edit ratio. Earlier electroporation resulted in a significantly lower mosaic edit to all edit ratio compared to later electroporation. *Denotes significant difference (p < 0.05) between groups.

Back to article page