Figure 1
From: Construction of a four-mRNA prognostic signature with its ceRNA network in CESC
The workflow of the present study. (A) First, we downloaded the clinical information and RNA-seq data of CESC patients from The Cancer Genome Atlas (https://portal.gdc.cancer.gov) (TCGA) database. (B) Second, we performed differential expression analysis between normal and tumor samples. (C) Then, we used univariate Cox regression analysis to identify the prognosis-associated mRNAs, miRNAs, and lncRNAs. (D) Then, functional annotation and pathway enrichment were performed to investigate the identified prognosis-associated mRNAs. (E) In addition, we constructed a ceRNA network based on these prognosis-associated RNAs. (F) Next, multivariate Cox regression analysis was used to screen the prognostic mRNAs for development of the prognostic model construction. (G) Finally, the prognostic model was developed using the 4 mRNAs selected by screening.
