Figure 1
From: Constitutive expression of a pea apyrase, psNTP9, increases seed yield in field-grown soybean
Identification of T-DNA copy number and insertion sites of transgenic soybean lines. (a) Southern blot analysis to determine the T-DNA copy number of soybean transgenic lines. Genomic DNA was digested with XbaI or BamHI, and a probe specific to the Bar gene was used to detect T-DNA insertions. (b) Diagrams of the sites of T-DNA insertions, including their adjacent genes, identified by TAIL-PCR and inverse PCR. Each of the inserted loci is shown as a chromosome number with the location in the chromosome. The locations of primers used for PCR validation of T-DNA insertion sites and the sizes of the resulting products are shown. Some parts of the diagram are not drawn to scale. (c–e) PCR validation of insertion sites for transgenic soybean lines. M: 1 Kb Plus DNA Ladder (Thermo Fisher Scientific), 1: negative control of non-transgenic soybean; 2, 3: transgenic plants of 14A (c), 17B (d), and 16C (e); –: distilled water control.
