Figure 5
From: Constitutive expression of a pea apyrase, psNTP9, increases seed yield in field-grown soybean
Transcript and protein abundance of psNTP9 of transgenic soybean lines. Transcript (a) and protein (b,c) abundance of psNTP9 is higher in leaves of the 17B line than in the 14A line of soybeans. The leaves were harvested from mature plants in the 2020 field trials in Shipman, IN. In panel (a) the relative expression levels were normalized to the expression level in 14A, taken as 1.0. Data represent means ± SE (n = 3). Different letters above the bars indicate statistically significant differences between the wild type and the transgenic lines using one-way ANOVA (p ≤ 0.01) with Tukey HSD test. In panel (b) equal loading was confirmed by dual staining with anti-actin antibodies. In panel (c) the lane outlined had less protein loaded than the following four lanes, as indicated by lower actin levels. 17B/DSR262 has a slightly higher level of psNTP9 immunostaining than 16C/A2835 and 16C/A2835, but all three have higher levels than line 14A/3431, which is below detection limit in this panel. In panel (b) the samples loaded were from the following events: 17B/AA3431 (lanes 1, 2, 3 and 6); 17B/D5R262 (lanes 4 and 5); homozygous line of 14A (lane 7); 14A/A3431 (lane 8). In panel (c) the samples loaded were from the following events: homozygous line of 14A (lane 1); 14A/3431 (lane2); null line (lane 3); 17B/A3431 (lane 4); 17B/D5R262 (lane 5); null line (lane 6); 16C/A2835 (lane 7); 16C/A2835 (lane 8). Original immunostained membranes for (b) and (c) did not detect bands below 25 kDa in any transgenic line, so images do not show this region of the blot (see Supplementary information).
