Figure 3 | Scientific Reports

Figure 3

From: Heatstroke-induced late-onset neurological deficits in mice caused by white matter demyelination, Purkinje cell degeneration, and synaptic impairment in the cerebellum

Figure 3

Purkinje cell number significantly decreased after heatstroke. To assess Purkinje cells, calbindin was immunostained in the brain and the calbindin-positive Purkinje cell numbers were counted. (a) Representative image of calbindin-immunoreactions in the cerebellum. Calbindin-immunoreactions stained the soma of Purkinje cells, the dendritic fibers that were widely stained in the molecular layer (ML), and the white matter. (b) Higher magnification images of the Purkinje cell layer in the control (CTL; left) group from 1 to 9 weeks after heatstroke. Purkinje cells in the CTL animals were aligned in an equidistant manner. However, the number of cells decreased after heatstroke. (c) The Purkinje cell numbers of the HS group were significantly decreased during the experimental periods after heatstroke (1, 3, and 9 weeks, n = 9, respectively) compared with the CTL group (n = 9). Data are expressed as means ± SD (*P < 0.05, Student’s t test). ML molecular layer, PL Purkinje cell layer, GL granular cell layer, WM white matter, SD standard deviation.

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