Figure 4 | Scientific Reports

Figure 4

From: Heatstroke-induced late-onset neurological deficits in mice caused by white matter demyelination, Purkinje cell degeneration, and synaptic impairment in the cerebellum

Figure 4

Immunostaining of postsynaptic density 95 in Purkinje cells after heatstroke. (a) Representative images of anti-postsynaptic density 95 (PSD95) (red), a postsynaptic marker, and calbindin (green) immunoreactions in the cerebellum in control mice. The PSD95 immunoreactions were observed in the Purkinje cells. Blue is due to 4′,6-diamidino-2-phenylindole nucleic staining. (b) The higher magnified images indicated that the PSD95-immunoreactions were located in the axon hillock of each Purkinje cell. The immunoreactions in the control group were widely recognized, such as plates, with relatively lower density in the Purkinje cells (arrowhead). The PSD95 immunoreactions 1 week after heatstroke (arrow) were gathered and concentrated in the axon hillock of the Purkinje cells like pen points. The immunoreactions appeared to have attenuated at 3 weeks and reappeared more heterogeneously at 9 weeks. ML molecular layer, PL Purkinje cell layer, GL granular cell layer, WM white matter, CTL control.

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