Figure 5
From: The stimulatory effect of fusobacteria on dendritic cells under aerobic or anaerobic conditions
The production of IL-6 by the DC line stimulated with live commensal bacteria. (A) Under aerobic (upper panel, white bar) and anaerobic (lower panel, black bar) stimulation conditions, TNF-α production during the initial (total of 6 and 8 h) and late phases (total of 24 h) by the DC line after stimulation with six strains of live commensal bacteria. (B) Under aerobic (white bar) and anaerobic (black bar) conditions, IL-6 production during the late phase (total of 24 h) by the DC line after stimulation with six strains of live commensal bacteria was assessed. The six live commensal bacterial strains are as follows: Fusobacterium varium (F. varium), Fusobacterium nucleatum (F. nucleatum), Bacteroides vulgatus (B. vulgatus), Clostridium clostridioforme (C. clostridioforme), Escherichia coli (E. coli), and Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus). The results are presented as the means ± SD from three independent experiments. Tukey’s multiple comparisons test was used to compare the levels of IL-6 after 6, 8, and 24 h of culture. ns; not significant, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
