Figure 4
From: Sulfation of sialic acid is ubiquitous and essential for vertebrate development
Intracellular localization of Wscd1 and Wscd2 in CHO cells. The V5-tagged Wscd1 and Wscd2 were immunostained with anti-V5 and Alexa-488-conjugated anti-chicken IgG (green) as the primary and secondary antibodies, respectively. Golgi apparatus is visualized with anti-GM130, followed by Alexa 555-conjugated anti-rabbit IgG (red). ER is visualized with anti-KDEL, followed by Alexa-594-conjugated anti-rabbit IgG (red). Nuclei are counter-stained with DAPI (blue). Co-immunostaining with anti-V5 and anti-GM130 (a, c), and anti-V5 and anti-KDEL (b, d) were performed.
