Figure 6
From: Sulfation of sialic acid is ubiquitous and essential for vertebrate development
Phenotypes of the Wscd1- or Wscd2-deficient medaka. (a, b) Survival curves for (−/−), (+/−), and (+/+) medaka in Wscd1 (a) or Wscd2 (b) gene, respectively. In-cross offspring of the heterozygous genotype medaka were used. (c) Impaired heartbeat of Wscd1(−/−) fry at 8 dpf. The circulating blood cells amount in the ventricular chamber was monitored in wild-type (WT), Wscd1(−/−), and Wscd2(−/−) fry for 10 s, analyzed by the Image-J. (d) Reduction in the amount of myosin heavy chain (MHC) in Wscd1(−/−) fry at 8 dpf. (Left panels) Western blotting of Wscd1(+/−) (upper panels) or Wscd2(+/−) (lower panels) using antibodies against MHC and β-actin (loading control). (Right panels) Values for the MHC/β-actin ratio. The error bars indicate the standard deviations from three independent data. *p < 0.05. (e) Growth retardation of Wscd2(−/−) fry at 50 dpf. Wscd2(+/+) and surviving Wscd2(−/−) fry (left) were analyzed for the weight (middle), and length (right). The number of fish are indicated by n. The error bars indicate the standard deviations. *p < 0.05, (Student t-test, n = 3).
