Figure 4

Gene expression profiles were greatly altered in Slc26a7^−/− mice. (a,b) Global patterns of gene expression in wild type (WT) (male n = 4), Slc26a7^−/− (male n = 4), and Slc26a4^−/− (male n = 3) mice are depicted using a MA plot with counts per million (CPM) and fold change (FC). Genes with a Q-value < 0.05 are indicated by red dots. The number of differentially expressed genes (DEGs) between WT and Slc26a7^−/− was 6371, and that between WT and Slc26a4^−/− was 37. (c) Pattern of expression of genes related to thyroid hormone synthesis is depicted using a volcano plot with FC and Q-value. Genes with ≥ 2-fold change and Q-value < 0.05 are indicated by pink dots. (d) Expression levels of the genes known to encode iodide transporters in male WT mice (n = 4) using the transcripts per million (TPM) value obtained by StringTie. One-way analysis of variance (ANOVA) showed significant differences among the seven groups (F (6, 21) = 20.0, p < 0.0001). ****p < 0.0001 determined by Tukey’s test. (e) Schema of the relationship between Slc26a7 and Slc26a4 in the thyroid follicular cell, as deduced from the results of this study. WT wild type, CPM counts per million, FC fold change, T3 triiodothyronine, T4 thyroxine, I⁻ iodide.