Figure 6
From: Alterations in rumen microbiota via oral fiber administration during early life in dairy cows

Exploration of the correlation between bacterial taxa and milk yield. (a) Heat map of hierarchical cluster analysis of rumen microbiota and milk yield. Rows represent the bacterial taxa (> 1.0% abundance) determined via 16S rRNA gene amplicon sequencing at 21 days after calving and average milk yield during the first 30 days of the lactation period. Columns represent the individual animals in each dietary group. Heat map color scale (black to green) displays the row Z-score (Z-score = [value of variable of an individual animal − mean of variable]/standard deviation). A dendrogram was generated based on Spearman’s correlation coefficient. (b) Relative abundance of the bacterial taxa clustered with milk yield in panel (a). Each box plot shows data of six animals and their range, median, and quartiles. Asterisk (*) and dagger (†) indicate significance (P < 0.05) and tendency (P < 0.10), respectively. P-values were calculated using a negative binomial model implemented as a Wald test in DESeq2 package in R software.