Figure 2
From: Evidence of impaired macroautophagy in human degenerative cervical myelopathy
(a) Differences in the total % area of LC3 staining in cases (n = 7) and controls (n = 4) excluding regions below 0.25 μm2 did not reach statistical significance. (b, c) Control sample LC3 staining of grey matter at high magnification. (d) Number of LC3 puncta per cell (regions 0.2–7.7 μm2 in size with a circularity over 0.99 divided by the number of nuclei counted by isolating nuclear staining) in the grey matter was significantly lower in cases (n = 7) than controls (n = 4). (e, f) LC3 staining of grey matter in one case at high magnification. Two-tailed t-tests were performed in (a). Two-tailed Mann–Whitney tests were performed in (d).
