Figure 5 | Scientific Reports

Figure 5

From: N(6)-methyladenosine methylation-regulated polo-like kinase 1 cell cycle homeostasis as a potential target of radiotherapy in pancreatic adenocarcinoma

Figure 5

METTL3 is expressed in a cell cycle-dependent manner similar to PLK1. (a) Is METTL3 regulated in a cell cycle-dependent manner? We hypothesized that METTL3 regulates cell cycle-dependent changes in PLK1 expression by methylating the 3′UTR of PLK1. (b) Flow cytometric analysis to observe the expression changes of METTL3 and PLK1 during the cell cycle. PLK1 is upregulated in the G2/M phase (upper middle and upper right). Similarly, the expression of METTL3 was slightly upregulated by 4 folds of that in DNA (lower middle) and G2/M phase (lower right), and the positive correlation with PLK1 expression was observed (lower left). (c,d) The expression of METTL3 (green) and PLK1 (magenta) was compared between the M phase and interphase using immunocytochemistry. The scale represents 20 µm. The fluorescence intensity of DAPI doubled in the M phase reflecting the amount of DNA, and the fluorescence intensity of PLK1 and METTL3 increased accordingly. Furthermore, a correlation was found between the fluorescence intensities of PLK1 and METTL3 (Pearson correlation, r = 0.64, p < 0.01).

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