Figure 2 | Scientific Reports

Figure 2

From: Identification of a differentiation-related prognostic nomogram based on single-cell RNA sequencing in clear cell renal cell carcinoma

Figure 2

Identification of ccRCC subsets by the single-cell trajectory analysis. (A) PCA of scRNA-seq data for preliminary dimensionality reduction. (B) tSNE was used for clustering of ccRCC cells. (C) Heatmap of marker genes in each cluster. Yellow represents upregulated genes and purple indicates downregulated genes. (D) Cell-type annotation of clusters. 24 clusters were divided into 9 cell types according to marker genes. (E) Single-cell pseudotime analysis of three subsets. Dark blue denotes an earlier time. (F, G) Trajectory analysis of clusters and cell types. The dots of different colors represented the corresponding clusters or cell types, which were arranged on the pseudotime branches.

Back to article page