Figure 3 | Scientific Reports

Figure 3

From: Preparation of recombinant glycoprotein B (gB) of Chelonid herpesvirus 5 (ChHV5) for antibody production and its application for infection detection in sea turtles

Figure 3

Verification of the specificity of anti-serum samples from mice immunized with various recombinant gB proteins. (A) Five antigenic fragments of gB protein and the fusion tag protein thioredoxin (Thx) were reacted with serum samples collected from mice immunized with F1, F2, F3, F1–2, and F2–3, as labeled on the top of each blot. The partial gB proteins specifically recognized by the mouse sera are indicated with asterisks. (B) Human 293 cells transiently expressing the full-length gB protein fused with a FLAG tag were used to confirm the specificity of antiserum F1, F2, F3, and F2–3. FLAG served as a positive control antibody against the gB-FLAG fusion protein. By IFA, the green fluorescence located in the cytoplasm indicated positive staining of gB. The nucleus was shown in blue by DAPI staining.

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