Table 6 ANOVA for expression levels of cytokines/chemokines-related genes or their receptors, which are selected by hypothesis, in THP-1 macrophages co-cultured with MMNK-1 cholangiocytes.

From: Transcriptome analysis of human cholangiocytes exposed to carcinogenic 1,2-dichloropropane in the presence of macrophages in vitro

Genes involved in intercellular signal

Regulation

Module eigengene

p-value for ANOVA

Mean ± SD of normalized values fold change

1,2-DCP concentration

0

0.1

0.4 mM

Cytokines

TNF Superfamily Member 4 (TNFSF4)

–

ME8

0.11

81 ± 16

1

52 ± 10

0.64

54 ± 19

0.67

Tumor necrosis factor alpha-induced protein 8-like protein 1 (TNFAIP8L1)

Downregulated

ME2

0.020

46.5 ± 8.4

1

27.3 ± 1.2

0.59 (0.013)

35.6 ± 5.6

0.77 (0.11)

Tumor necrosis factor, alpha-induced protein 8-like 2 and sodium channel modifier 1 (TNFAIP8L2-SCNM1)

–

ME3

0.15

150 ± 35

1

215 ± 47

1.43

200 ± 24

1.33

TNF Receptor Superfamily Member 10a (TNFRSF10A)

–

ME3

0.07

11.4 ± 4.1

1

14.7 ± 6.7

1.28

28 ± 10

2.42

Chemokines

C–C motif chemokine ligand 2 (CCL2)

Upregulated

ME3

0.018

436 ± 83

1

575 ± 42

1.32 (0.057)

636 ± 52

1.46 (0.013)

C–C motif chemokine ligand 7(CCL7)

–

ME7

0.096

29.3 ± 6.5

1

33 ± 11

1.11

51 ± 13

1.73

C-X-C motif chemokine ligand 2 (CXCL2)

Downregulated

ME2

0.0042

27.8 ± 2.6

1

16.1 ± 4.4

0.58 (0.018)

10.6 ± 4.4

0.38 (0.0028)

C-X3-C motif chemokine receptor 1(CX3CR1)

Upregulated

ME2

0.0016

13.5 ± 5.5

1

30.6 ± 6.2

2.26 (0.024)

32.3 ± 6.4

2.39 (0.016)

C–C motif chemokine receptor like 2(CCRL2)

–

ME9

0.053

35 ± 13

1

50 ± 12

1.46

62.8 ± 7.1

1.82

C–C motif chemokine receptor 6(CCR6)

Downregulated

ME2

0.0083

30.7 ± 1.0

1

17.9 ± 4.5

0.58 (0.0074)

19.8 ± 3.9

0.64 (0.015)

  1. Normalized values of expression level were compared between three groups of different 1,2-DCP concentration by one-way analysis of variance (ANOVA), being followed by post hoc Dunnett’s multiple comparison with control (0 mM 1,2-DCP group). Data represents expression levels of cytokines/chemokines-related genes or their receptors from transcriptomic profiles of THP-1 macrophages co-cultured with MMNK-1 cholangiocytes when exposed to 1,2-DCP at 0, 0.1 or 0.4 mM for 24 h. Data of normalized values for gene expression are mean ± SD, n = 3. Fold change represents the value relative to the average of the control group (0 mM).
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