Figure 1 | Scientific Reports

Figure 1

From: Human chorion-derived mesenchymal stem cells suppress JAK2/STAT3 signaling and induce apoptosis of cholangiocarcinoma cell lines

Figure 1

CH-CM increases the inhibition of the proliferation rate of CCA cell lines. (A) Three CCA cell lines were treated with CH-CM (0%, 10%, 25%, 50% and 75%). Every 24 h for 5 days, cell proliferation was measured by MTT assay. CH-CM significantly increased the proliferation inhibition rate of all three CCA cell lines. (B, C) Induction of apoptosis by CH-MSCs conditioned media in CCA cell lines. After culturing CCA cells with CH-CM (0%, 50%, 75%) for 12 h and 24 h, apoptotic cells were stained with annexin V-PI. Flow cytometric analysis showed that the number of apoptotic cells significantly increased in a dose-dependent manner compared with the control group. All data are presented as the mean ± SEM (n = 3) of a minimum of 3 donors. *p < 0.05, **p < 0.01, ***p < 0.001 compared to the control group.

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