Figure 3

CH-CM induces activation of caspase-3 activity in CCA cell lines. The caspase-3 activity was determined by a microplate reader after culturing CCA cell lines with CH-CM (0%, 50%, 75%) for 8 h. The results showed that the caspase-3 activity in CCA cell lines was significantly higher after being cultured with CH-CM compared with control. Data are expressed as mean ± SEM; n = 3 **p < 0.01, ***p < 0.001 versus control.