Figure 2
From: Microfluidic model for in vitro acute Toxoplasma gondii infection and transendothelial migration
Tachyzoite infection model with a 3D EC monolayer in the microfluidic platform. (a) Time line of 3D EC monolayer formation and tachyzoite infection. (b) Phase images of EC monolayer at days 5 and 2. (c) Confocal image of the 3D EC monolayer on day 0 before seeding the tachyzoites. (d) Sequential phase images with 12 h intervals after the infection of tachyzoites. Infected cells indicated by arrowheads show the cycle, from duplication to egress. Scale bar indicates 100 µm. (e) Area fraction of tachyzoite in the EC monolayer and ECM region. (n = 8 for each group). (f) Overview of the tachyzoite cycle within the EC monolayer. Scale bar indicates 50 μm. Statistical significance was analyzed by one-way ANOVA with Holm-Sidak method (e) and is indicated by asterisks as follows: *P < 0.05, **P < 0.01, ***P < 0.001.
