Table 4 Standard procedure to prepare pyruvate samples.
From: Performance and reproducibility of 13C and 15N hyperpolarization using a cryogen-free DNP polarizer
Step | Operation | Duration | Comments | Standard settings |
|---|---|---|---|---|
1 | Take one aliquot of around 1.5 mL of 1-13C-PA and one aliquot of around 50 mg of trityl radical out of the fridge | Before use store at − 20 °C | ||
2 | Wait until the pyruvic acid warms up and becomes liquid | 20 min | Keep the vial in a warm place. Avoid direct sunlight | |
3 | Put 25.8 mg of trityl radical in a vial | e.g. Eppendorf tubes | ||
4 | Add 0.68 g of 1-13C-PA into the vial | |||
5 | Mix it | 10 min | Centrifuge the vial | |
6 | Divide into c.a. 250 µL aliquots | e.g. Eppendorf tubes | ||
7 | Put in the freezer | Store at − 20 °C |
