Figure 2
From: CRISPR/Cas9-based genome-wide screening of Dictyostelium
Generation of a genome-wide and kinase sgRNA plasmid library. (a) Target gRNA positions in the genome-wide and kinase libraries. Target sequences in genome library were designed within 400Â bp of the start codon, while kinase libraries were designed with five gRNAs per 1Â kb of each gene. Three representative genes are shown. The kinase domain of each gene is indicated by an orange box. (b) Distribution of designed gRNAs in genes. (c) Deep-sequencing analysis of the gRNAs in plasmid libraries and genome-wide (left) and kinase (right) mutant pools.
