Figure 5
From: CRISPR/Cas9-based genome-wide screening of Dictyostelium
In-frame indel mutations in tor identified in the screens. (a) Developmental phenotypes of in-frame indel mutations in tor cells. The cells were plated on non-nutrient agar and imaged. Scale bars, 250 µm. (b) Chemoattractant-mediated activation of PKBR1 in 224 N tor mutants. Phosphorylated PkbR1 was analysed by immunoblotting and the band intensity was quantified. Mean values from three independent experiments and individual data points are shown. Error bars represent the SEM.
