Figure 4
NIS protein and 125I uptake in cancer cells treated with EVs. (A) Western blots of NIS protein obtained from the plasma membrane fractions of recipient Huh7 liver cancer cells treated with EVs derived from Huh7/NIS-Adv and MDAMB/NIS-stable donor cells (n = 2 per group). (B) Time dependence of 125I uptake in recipient Huh7 cells treated with EVs s derived from Huh7/NIS-Adv cells. (C) 125I uptake in recipient Huh7 and HepG2 human liver cancer cells, and T47D human breast cancer cells in 24-well plates at 48 h after treatment with 25 µg per well of EVs derived from Huh7/NIS-Adv or MDAMB/NIS-stable donor cells. All cell uptake data are the mean ± SD of values from triplicate samples per group with or without perchlorate inhibition. *P < 0.05; **P < 0.01; ‡P < 0.0001, compared to controls.
