Figure 5
Ex vivo biopsies stained for IL-33. Colonic biopsies from healthy controls showing immunoenzymatic staining for IL-33 (brown) and cell nuclei counterstained with hematoxylin (blue). (A–C) shows biopsies at: baseline 0 hours (A) 12 h (B) and 24 h (C) with stimulation with TGF-beta-1 10 ng/ml. Sporadic but positive epithelial cells for IL-33 were seen. Images (D,E) also show healthy controls after 24 h of TGF-beta-1 stimulation. Image (H) shows stimulation with proinflammatory cytokine TNF 10 ng/ml for 24 h, with no positive epithelial cells for IL-33, also of note IL-33 in endothelial vessels was lacking (arrows). Scale bars = 20 µm. Graphs (F) and (G) shown results from image quantification of positive cells.
