Figure 7
Immune analysis of TBC1D8. (A) Effects of high and low TBC1D8 expression on immune cells. (B) Relationship between TBC1D8 level and degree of immune infiltration, from TIMER. (C,D) Relationship between TBC1D8 and levels of (C) M2 macrophage markers and (D) M1 macrophage markers. (E) Tumor-initiating cell proportions in CRC samples. from GSE10950 and GSE37182 data based on CIBERSORT. (F) Relationship between TBC1D8 level and macrophage abundance based on CIBERSORT, from GSE10950 and GSE37182 data. (G) Diagram of co-culture system. (H) Double-immunofluorescence staining of M2 macrophage markers CD206 (red) and CD163 (green); nuclei are stained with DAPI (blue) (Magnification, × 400, scale bars = 20 μm) (n = 3 replicates). (I) Intensity of immunofluorescence (mean ± SEM) (n = 3 replicates). Data are means ± SEM *p < 0.05; **p < 0.01; ***p < 0.001.
