Figure 8
The genetic alteration of TBC1D8 in CRC. (A) Frequencies of TBC1D8 mutations and copy number alterations (CNA) in the 8 datasets shown on the right side. (B) Volcano map of genes showing differential expression after a change in TBC1D8 (mutated and wild). Red dots, upregulated genes; blue dots, downregulated genes; abscissa, differences in gene expression (log2 fold change); and ordinate, significance of these differences (− log10 padj). (C) The mutation site profile of the TBC1D8 gene is shown. (D) GSEA was used to determine the functions of differential gene sets between the mutated and wild groups based on GO. Only gene sets with Normal P < 0.05 and FDR < 0.1 were considered significant and displayed in the plot. The x axis represents the distribution of log-fold change (logFC) corresponding to the core molecules in each gene set. (E–F) Differential Analysis of TBC1D8 (mutated and wild) with M1 (E) and M2 (F) macrophage markers. Wilcoxon-Mann–Whitney test was performed based on TIMER database. (G) The Differential Analysis of the TBC1D8 probe methylation were indicated. (H) Waterfall plot of the methylation levels in the TBC1D8 gene. The correlations between TBC1D8 methylation or expression levels were also analyzed. (I) Venn diagram showing the intersection of (G,H). (J) Survival analysis based on the intersection methylation probes; P < 0.05 was considered statistically significant.
