Table 1 qRT-PCR Primer sequences. Detailed forward and reverse primer sequences used for qRTPCR are provided.

From: Prior exposure to microcystin alters host gut resistome and is associated with dysregulated immune homeostasis in translatable mouse models

Genes

Primer sequence (5′-3′ orientation)

Toll-like receptor 2 (TLR2) [Mus musculus]

Forward: ACCAAGATCCAGAAGAGCCA

Reverse: CATCACCGGTCAGAAAACAA

Toll-like receptor 4 (TLR4) [Mus musculus]

Forward: GGAGTGCCCCGCTTTCACCTC

Reverse: ACCTTCCGGCTCTTGTGGAAGC

Regenerating islet-derived protein 3 gamma (REG3G)

[Mus musculus]

Forward: CCCGTATAACCATCACCATCAT

Reverse: GGCATAGCAATAGGAGCCATAG

Cluster of Differentiation 28 (CD28)

[Mus musculus]

Forward: ATGTACCCTCCGCCTTACCT

Reverse: CCACTGTCACTAGCAAGCCA

Cluster of Differentiation 57 (CD57)

[Mus musculus]

Forward: GGGTCATCTCTGGGTCATCC

Reverse: TGCCCCTCTGAAGAACCAAC

Interleukin-7 (IL-7)

[Mus musculus]

Forward: TGCTGCTCGCAAGTTGA

Reverse: GTGCAGTTCACCAGTGTTTG

Programmed cell death protein 1 (PD1)

(Mus musculus)

Forward: CCCTAGTGGGTATCCCTGTATT

Reverse: TCCTTCAGAGTGTCGTCCTT

18S

[Mus musculus]

Forward: TTCGAACGTCTGCCCTATCAA

Reverse: ATGGTAGGCACGGCGATA

Cluster of Differentiation 28 (CD28)

[Homo sapiens]

Forward: CCCTTCACAAAGGACTGGATAG

Reverse: CATTGCCCAATTTCCCATCAC

Cluster of Differentiation 57 (CD57)

[Homo sapiens]

Forward: ATCCTAGCGATCGTCCTCAT

Reverse: GTCACTGCCCTCATCCTTATG

18S

[Homo sapiens]

Forward: CGGCGACGACCCATTCGAAC

Reverse: GAATCGAACCCTGATTCCCCGTC

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