Figure 4
From: Efficacy of auranofin as an inhibitor of desmoid progression
Effects of auranofin on expression of β-catenin and downstream genes of Wnt/β-catenin signaling pathway. (A) Effects of auranofin (0–10 µM) on the expression of β-catenin protein. The T41A-mutated cells were seeded on a 6-well plate (1 × 105 cells per well) for 12 h followed by auranofin (0–10 µM) treatment for 24 h. These cells were subjected to western blotting using Simple Western for β-catenin and β-actin. (B) Fluorescent immunostaining for β-catenin. The T41A-mutated cells were seeded onto chamber slides (1.0 × 105 cells/ml) for 12 h followed by auranofin treatment (0–5 µM) for 24 h. Cells were fluorescently visualized using mouse anti-β-catenin antibody. (C) Expression levels of mRNA, AXIN2, CCND1, MYC, PTGS2. The DF cells (WT, T41A and S45F: 1 × 104 cells per well) were seeded in a 96-well plate for 12 h. Subsequently the cells were cultured in a medium containing felodipine, meloxicam, and auranofin (5 μM) for 6 h, and subjected to RT-PCR. CTL: control. (D) Effects of auranofin (0–5 µM) on the mRNA expression of four TCFs. The T41A cells (1 × 104 cells per well) were seeded in a 96-well plate for 12 h. Subsequently the cells were cultured in a medium containing auranofin (0–5 µM) for 6 h, and subjected to RT-PCR. *P < 0.05, **P < 0.01.
