Figure 13

Relative IL-1β, IL-6, IL-10, and TGF-β mRNA expressions were measured by qRT-PCR using the 2_ΔΔCt method and b-actin as an internal control. The administration of ointments based on malachite reduced the expression of IL-1β and IL-6 compared to the control group. The administration of ZnO/Mlt and ZnO/Mlt/Chn ointments increased the expression of IL-10 on days 3 and 7 and TGF-β on day 7 compared to the control group. The lowest expression for IL-1β and the highest expression for IL-10 were observed in the mice treated with ZnO/Mlt/Chsn ointment and antibiotic. All data are presented in the mean ± SD. The different letters above each column indicate a difference in level (P < 0.05) per sampling day.