Figure 3 | Scientific Reports

Figure 3

From: Lack of Cathepsin D in the central nervous system results in microglia and astrocyte activation and the accumulation of proteinopathy-related proteins

Figure 3

Increase of autophagy-related proteins expression in thalamus of CtsD-CKO mice. Immunohistochemical staining of ubiquitin (Ub) (A,B), p62 (D,E), LC3 (G,H) and Nbr1 (J,K) in thalami of CtsD-CKO (B,E,H,K) and control littermate (A,D,G,J) mice at p25. Insets show enlarged boxed areas of neuronal cells in the thalamus of CtsD-CKO that possess immuno-positive granules (arrows). Scale bar; 20 µm. The numbers of cells possessing ubiquitin (C), p62 (F), LC3 (I) and Nbr-1 (L) positive granules in the thalamus were quantified. Immunostained brain sections form CtsD-CKO mice were compared with age-matched control littermate mice. (**p < 0.03, ***p < 0.001, from p19 to p25, All data were obtained from three sections form 3 separate mice. Over 72 cells per each section were estimated).

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