Figure 5
Effective establishment of cocaine self-administration model for rats. (A, B, D, E) The cocaine self-administration training everyday. (A) Traditional Operation + Saline Training group (n = 15): Compared to the first three days, the number of valid nose-poke for the last three days decreased [Day1–3: 9.9333 ± 1.002, Day12–14: 1.222 ± 0.2480, tTO+ST group = 8.243, P < 0.01 (a)] during saline SA training period and reached a relatively stable level during the last three days of training period (FTO+ST group = 2.587, P = 0.1180). (B) Traditional Operation + Cocaine Training group (n = 13): Compared to the first three days, the number of valid nose-poke for the last three days increased significantly [Day1–3: 8.744 ± 1.390, Day12–14: 31.05 ± 1.6272, tTO+CT group = 11.42, P < 0.01 (b)] during cocaine SA training period and kept relatively stable during the last three days of training period (FTO+CT group = 2.616, P = 0.0946). (C) The number of valid nose-poke for the last three days between TO + CT group and TO + ST group was significant difference (Day12–14 in TO + ST group: 1.222 ± 0.2480, Day12–14 in TO + CT group: 31.05 ± 1.6272, tTO+CT group, TO+ST group = 19.41, P < 0.01). (D) Optimized Operation + Saline Training group (n = 28): Compared to the first three days, the number of valid nose-poke for the last three days decreased [Day1–3: 9.1786 ± 0.5814, Day12–14: 1.381 ± 0.1561, tOO+ST group = 13.05, P < 0.01 (d)] during saline SA training period and reached a relatively stable level during the last three days of training period (FOO+ST group = 6.146, P < 0.01). (E) Optimized Operation + Cocaine Training group (n = 28): Compared to the first three days, the number of valid nose-poke for the last three days increased significantly [Day1–3: 10.83 ± 0.8865, Day12–14: 31.89 ± 1.5945, tOO+CT group = 11.71, P < 0.01 (e)] during cocaine SA training period and kept relatively stable during the last three days of training period (FOO+CT group = 0.2034, P = 0.7714). F. The number of valid nose-poke for the last three days between OO + CT group and OO + ST group was significant difference (Day12–14 in OO + ST group: 1.381 ± 0.1561, Day12–14 in OO + CT group: 31.89 ± 1.5945, tOO+CT group, OO+ST group = 19.04, P < 0.01). TO + ST group Traditional Operation + Saline Training group, TO + CT group Traditional Operation + Cocaine Training group, OO + ST group Optimized Operation + Saline Training group, OO + CT group Optimized Operation + Cocaine Training group. Data were presented as means ± SEM, **P < 0.01.
