Figure 1
From: Induction of potassium channel regulator KCNE4 in a submandibular lymph node metastasis model
Selection of SLNs before melanoma metastasis for the analysis of early metastasis. (A) Schedule of sample preparation for microarray analysis. (B) Macroscopic images of the tongue and SLNs on day 3 after transplantation. Ctrl is a mouse injected with PBS and #1–8 are mice injected with B16–F10 into the tongue. LNs in which melanoma had metastasized turned black, as indicated by the arrowheads. (C) LNs #1, 3, and 8 with no metastasis and #4 with metastasis in macroscopic images were stained with Giemsa. Melanin pigment was detected as shown by the arrowhead in #4. Bars: 100 µm and 50 µm. (D) Mlana expression was examined by qRT-PCR in the right-hand LNs of mice #1–8 transplanted with B16–F10. Relative expression levels were adjusted for Actb expression. One-way ANOVA, ***p < 0.001 versus ctrl.
