Figure 2
From: Induction of potassium channel regulator KCNE4 in a submandibular lymph node metastasis model
Genes with altered expression in SLNs during early metastasis. (A) Annotation analysis of genes whose expression had changed (B16–F10 vs control, fold change of < − 2 and > 2, respectively; p < 0.05). (B) Volcano plot of genes with altered expression. (C) Verification of Kcne4, Slc7a11, Fscn1, and Gadd45b expression by qRT-PCR. The expression levels of target genes in SLNs of control mice and SLNs of mice implanted with B16–F10 (right and left) were analyzed. One-way ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001. ns; not significant. (D) LECs were co-cultured with B16–F10 in transwell plates for 24 h, and the expression levels of Kcne4, Slc7a11, Fscn1 and Gadd45b were analyzed by qRT-PCR. ***p < 0.001. ns; not significant.
