Figure 3
From: Induction of potassium channel regulator KCNE4 in a submandibular lymph node metastasis model
KCNE4 is upregulated by melanoma transplantation and is expressed on podoplanin-positive cells in SLNs. Expression of KCNE4 in SLN of mice transplanted with B16–F10 was examined by immunohistochemistry. Double staining was performed with anti-KCNE4 antibody and anti-CD45 antibody (A), anti-podoplanin antibody (B), and anti-PNAd antibody (C). Lymph nodes from mice injected with PBS were used as controls. Bars: 200 µm and 50 µm. (D) The percentages of KCNE4-positive areas in SLNs of ctrl or B16–F10 transplanted mice were measured by ImageJ software and divided by the area of the SLNs. Student’s t test, **p < 0.01.
