Figure 2

Genomic analysis of cirrhosis and orthotopic HCC tumors. (A) Transcriptome data of cirrhotic mouse tissue acquired by RNAseq were compared against the Hallmark gene sets (MSigDB), highlighting upregulation of numerous growth factor and inflammatory pathways (e.g., IL6, KRAS, TNFα) commonly seen in human cirrhosis. (B) Transcriptome data of orthotopic HCA-1 mouse tumor tissue acquired by RNAseq were compared to previously published human HCC tumors (Gene Expression Omnibus accession GSE14520); mouse tumors were found to cluster with advanced human HCC tumors (CLIP score > 2). (C) Gene set enrichment analysis of the HCA-1 cell line transcriptome revealed a strong similarity with the previously published gene expression profile for HCC tumors classified by gain-of-function mutations of CTNNB1, a molecular subclassification characterized by an “immune desert” phenotype.