Figure 4
Immunohistochemical analysis of orthotopic HCC tumors treated with MTPA. MTPA was performed in an orthotopic mouse model of HCC with underlying cirrhosis. (A) IHC of CD8+ cells demonstrates minimal infiltrating T cells in control tumors but a substantial increase following anti-PD1 therapy combined with MTPA (red bar = 100 mm). (B) Quantitative analysis of IHC data (error bars reflect standard error) reveals a significant increase in intratumoral CD3+ and CD8+ T cells following MTPA alone and MTPA+anti-PD1 therapy, along with a decrease in CD4+FOXP3+ Tregs. Percentages in panel B reflect the proportion of positively stained cells within a region of interest circumscribing the tumor. Box plots reflect mean +/− standard error. The non-parametric Mann–Whitney U-test was used to evaluate differences in the proportions across treatment groups.
