Figure 3
Vaccine-induced antigen-specific antibody (IgG and IgA) secreting memory B-cell responses performed by ELISpot assay. PBMCs collected from vaccinated subjects on Day 215 were pre-activated or pre-stimulated with polyclonal expansion using Poly B stimulant for 4 days, with unstimulated cells or cells without pre-activation as negative controls. Antibody-secreting cells (ASC) were detected with anti-human IgG (biotin) and anti-human IgA (FITC) antibody followed by streptavidin–ALP and FITC-HRP respectively. Boxes indicate upper and lower quartiles, lines within the box indicate median and whiskers extending from the boxes indicate the upper and lower quartiles.
