Table 1 Differential phenotypic characteristics that distinguish strain AK-R2A1-2 T from closely related type strains. Strains: 1, AK-R2A1-2 T; 2, Subtercola lobariae KCTC 33586 T; 3, Subtercola frigoramans KCTC 49696 T; 4, Subtercola boreus DSM 13056 T; 5, Subtercola vilae DSM 105013 T. All data are from the present study unless indicated otherwise. + , Positive; w, weakly positive; − , negative; ND, not detected.
From: Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana
Characteristic | 1 | 2 | 3 | 4 | 5 |
|---|---|---|---|---|---|
Isolation source | Abies koreana | Lichena | Boreal groundwaterb | Boreal groundwaterb | Cold volcano lakec |
Colony color | White, circular | White, circulara | Pale yellow to bright yellowb | Yellow, circularb | Golden yellowc |
Cell size (µm) | (0.2–0.3) × (0.3–1.6) | (0.5–0.6) × (0.7–1.0) a | (0.3–0.4) × (0.9–1.5)b | (0.2–0.3) × (0.6 − 1.0)b | 0.5 × (1.0–1.2)c |
Growth conditions | |||||
Temperature range (optimum)(°C) | 4–25 (20) | 4–28 (20)a | 2–28 (15–17)b | 2–28 (15–17) b | 5–28 (10–15)c |
NaCl tolerance (optimum) (%, w/v) | 0–1 (0) | ND | ND | ND | 0c |
pH range (optimum) | 5.0–8.0 (5) | 4.0–8.0 (6)a | ND | ND | 5.0–8.0 (7)c |
Major menaquinone | MK-9, MK-10 | MK-10a | MK-9, MK-10b | MK-9, MK-10b | MK-9, MK-10c |
G + C content (mol%) | 65.8 | 66.8a | 67.8b | 64c | 65d |
API 20NE | |||||
Indole production | − | − | − | + | − |
Esculin hydrolysis | + | + | + | w | + |
β-Galactosidase | + | − | + | − | − |
Glucose assimilation | − | − | + | − | − |
API ZYM | |||||
Alkaline phosphate | − | − | + | − | w |
Esterase(C4) | + | + | + | + | w |
Lipase (C14) | w | − | − | w | − |
Valine arylamidase | w | w | w | − | w |
Cystine arylamidase | w | w | w | − | − |
Trypsin | w | − | w | − | − |
β-Galactosidase | w | − | + | + | − |
α-Mannosidase | w | + | − | − | − |
API 50CH | |||||
Glycerol | − | − | − | − | w |
D-Arabinose | − | − | − | − | w |
L-Arabinose | − | − | − | − | w |
D-Xylose | − | − | w | − | w |
L-Xylose | − | − | w | w | w |
D-Adonitol | − | − | w | w | w |
Methyl-βD-xylopyranoside | − | − | w | w | w |
D-Galactose | − | − | + | w | |
D-Glucose | − | − | w | − | w |
D-Fructose | − | − | − | − | w |
L-Sorbose | − | − | − | − | w |
L-Rhamnose | − | − | − | − | w |
Inositol | − | − | − | − | w |
D-Mannitol | − | − | − | − | w |
N-Acetylglucosamine | − | − | − | w | − |
Amygdalin | − | − | w | w | − |
Salicin | − | − | w | w | w |
D-Cellobiose | − | − | w | w | w |
Maltose | − | − | w | w | w |
D-Lactose | − | − | + | w | w |
D-Melezitose | − | − | w | + | + |
D-Saccharose | − | − | w | − | − |
D-Trehalose | − | − | w | − | − |
Gentiobiose | − | − | − | − | w |
Potassium 2-keto-gluconate | − | − | − | − | w |
