Figure 7
The FAM83H mutants used in the study. (a) The full length of FAM83H is composed of 1179 amino acid residues. Light gray columns indicate the amino acid residues of FAM83H. Dark gray columns indicate the amino acid residues of the FLAG-tag. Green columns indicate the amino acid residues of EGFP. Red lines indicate the position of the keratin-localization (KL) residues (1134–1139). The open circle and cross marks indicate the positive and negative localizations of FAM83H and the recruitment of CK1α to the keratin cytoskeleton (K) or nuclear speckles (NS) in DLD1 cells, respectively. The dash mark indicates that was not tested. (b) Schema. FAM83H mutants with the KL residues localize and recruit CK1 to the keratin cytoskeleton, whereas mutants without the KL residues localize and recruit CK1 to the nuclear speckles.
