Table 1 Concordance between microbial genus-level detection by quantitative bacteriology and metagenomic sequencing.

From: Metagenomic features of bioburden serve as outcome indicators in combat extremity wounds

Genus

QuantBAC Prevalence

Metagenomic sequencing percent positive agreement

Overall

65/66 (98.5%)

54/65 (83.1%) [72.2–90.3%]

Acinetobacter

38/66 (57.6%)

38/38 (100%) [90.8–100%]

Enterococcus

14/66 (21.2%)

7/14 (50%) [26.8–73.2%]

Achromobacter

5/66 (7.6%)

2/5 (40%) [11.8–76.9%]

Pseudomonas

2/66 (3%)

2/2 (100%) [34.2–100%]

Staphylococcus

2/66 (3%)

2/2 (100%) [34.2–100%]

Bacillus

1/66 (1.5%)

1/1 (100%) [20.7–100%]

Citrobacter

1/66 (1.5%)

1/1 (100%) [20.7–100%]

Enterobacter

1/66 (1.5%)

1/1 (100%) [20.7–100%]

Escherichia

1/66 (1.5%)

1/1 (100%) [20.7–100%]

  1. Concordance was assessed according to prevalence, with positive percent agreement estimates used to compare genus detection from metagenomic sequencing to quantitative bacteriology, applied here as a non-reference standard. Note: except for one case, these tests did not have a Negative result to tabulate.
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