Figure 1 | Scientific Reports

Figure 1

From: Mouse oocytes carrying metacentric Robertsonian chromosomes have fewer crossover sites and higher aneuploidy rates than oocytes carrying acrocentric chromosomes alone

Figure 1

MPI progression in the oocytes of RbT-carrier and WT mouse ovaries at 15.5–17.5 dpc. (a) MPI substages identified by SYCP3 immunofluorescence (IF) staining. In Rb5 and RBF oocytes, RbT-centromeres (indicated by arrowheads) were identified by IF-staining of centromeres with CREST antibody (red) without adjacent telomeres with anti-TRF1 antibody (green). C- and D-telomeres were also distinguished by IF-staining of TRF1 with and without adjacent CREST staining, respectively. Scale bar 10 μm. (b) Percentages of oocytes at progressive MPI substages in the ovaries of each genotype. The number of examined oocytes and females (in parentheses) are given on the top of each column. *Significant difference between WT and Rb5 or RBF ovaries at P < 0.05 by χ2-test.

Back to article page