Table 2 Optimization of the reaction conditions.
From: Biocatalytic decarboxylative Michael addition for synthesis of 1,4-benzoxazinone derivatives
Entry | Solvent | Temperature (°C) | Enzyme loading (mg) | Water (µl) | Time (h) | Yielda (%) |
|---|---|---|---|---|---|---|
1 | DMSO | r.t | 10 | 20 | 24 | n.r |
2 | DMF | r.t | 10 | 20 | 24 | n.r |
3 | THF | r.t | 10 | 20 | 24 | 10 |
4 | DCM | r.t | 10 | 20 | 24 | 15 |
5 | MeCN | r.t | 10 | 20 | 24 | 45 |
6 | MeCN | 40 | 10 | 20 | 24 | 55 |
7 | MeCN | 50 | 10 | 20 | 24 | 57 |
8 | MeCN | 60 | 10 | 20 | 24 | 43 |
9 | MeCN | 40 | 20 | 20 | 24 | 65 |
10 | MeCN | 40 | 30 | 20 | 24 | 70 |
11 | MeCN | 40 | 40 | 20 | 24 | 70 |
12 | MeCN | 40 | 30 | 30 | 24 | 72 |
13 | MeCN | 40 | 30 | 40 | 24 | 75 |
14 | MeCN | 40 | 30 | 50 | 24 | 75 |
