Figure 2
From: Paternal age impairs in vitro embryo and in vivo fetal development in murine
In vitro embryo development. Image and chart of cleaved embryos from the young and senile group (number of cleaved embryos/number of total structures × 100; a). Image and chart of blastocyst from the young and senile group (number of blastocysts/number of total structures × 100; b), and embryo development rates (number of blastocysts/number of cleaved embryos × 100; c) from young and senile groups. In the young group: 185 total structures (presumed zygotes) from 6 pregnant dams (30.8/pregnant dam), 122 cleaved embryos from 6 pregnant dams (20.3/pregnant dam), and 61 blastocysts from 6 pregnant dams (10.1/pregnant dam). In senile group: 107 total structures from 5 pregnant dams (21.4/pregnant dam), 27 cleaved embryos from 5 pregnant dams (5.4/pregnant dam) and 3 blastocysts from 5 pregnant dams (0.6/pregnant dam). The evaluations were performed in stereomicroscope (Olympus SZ61, Olympus®, Tokyo, Japan) at ×60 magnification. Different superscript letters in each bar represent p < 0.05, as indicated by statistical T-test.
